I have some basic data analysis questions:
- What do you take into account when selecting the fluorescence value threshold for spike activity, and what would make a good threshold? (somewhere between mean and max?)
- In the ROI selection of msBatchSelectROI, should you select single neurons as ROIs (multiple ROS's) or do you select the general area you want to align (1 to 3 ROIs)?
I know the answers to these questions can vary based on what one tries to analyze, in my case I'm looking to increase the (neuron)signal to (background)noise ratio when trying to isolate single neuron dF/F.
|Posted by Jesper.vernooij on 14 March 2016 at 18:22.|
Edited by Jesper.vernooij on 14 March 2016 at 18:22.