Forum - Behavior FAQ

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Please use this forum to discuss the process of running behavior using our miniscope system. Please see our Animal Behavior Guide for an introduction to the process of imaging during behavior.

Posted by Tristanshuman (administrator) on 14 January 2016 at 16:50.

Hi everybody, I am thinking of using this Miniscope for head-fixed mice OFC imaging. Do you see any advantage with head-fixed mice with more concise set-up with Miniscope, or more stable data collection or something like that? Or do you think there would be better method with head-fixed mice?

Thank you, Masaru

Posted by Masaru on 27 May 2016 at 21:27.

I have used head fixed mice to image visual cortex through a cranial window using a two photon laser calcium microscope, which went very well and produces high-quality and stable imaging, the price tag however is daunting. Because of the price tag you could try first with the miniscope. Another advantage is that using a Grin lens with the miniscope might give you more mobility when getting to the OFC, when compared to a rigid two photon setup.

Posted by Jesper.vernooij on 3 June 2016 at 18:17.

Thank you Jesper for reply. I heard OFC is too deep to do two photon imaging, but could be a option if it's possible with Grin lens or something like that. What do you mean by mobility? Actually my concern was that we can get only one position for the imaging out of fixed Grin lens but do you think we can have multiple location by advancing lens little by little?

Posted by Masaru on 3 June 2016 at 21:21.