Hi, below a list of questions regarding the procedures:
a. For the virus injections, how many uL do you usually inject and what is a common titer of your viruses? Do you dilute them at all? According to the recent protocol by Stuber lab, they almost always dilute them (at least 1:1), but in other papers I don't see any mention of dilution.
Do you do any long-term (i.e. 1-2 months after injection) recordings (in which case, according to the above protocol the high concentration can be problematic)?
b. When implanting the GRIN lens, do you lower it until it touches the surface of the hippocampus or is it fine if there is air/saline in between? I see in the wiki that you lower it 1.35mm, but I suppose this is dependent on the exact coordinates of the craniotomy, so I am trying to identify the general strategy.
c. According to the Stuber lab protocol, they cover everything with Black Cement. Do you also do this step?
d. In the wiki the steps for implanting the baseplate have not been uploaded yet, but is the general idea similar to the way described in the Stuber lab protocol (Fig. 14)? If I understand correctly, the procedure is done in a separate surgery because it needs to be done with the miniscope attached while observing the cells/FoV?
e. Are you planning to upload in the near future any videos of the surgery procedure?
|Posted by Nikolas on 25 March 2016 at 17:28.|