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Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
 
Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:
*All nonessential componets have been removed
+
* All nonessential components are removed
*The table top objective was replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
+
* The table top objective is replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
*The miniature fluorescence microscope does not use any infinity optics
+
* The excitation light source is a super bright LED rather than a lamp or laser
*The excitation light source is a super bright LED rather than a lamp or laser
+
* The optical filters and dichroic mirror are custom cut to minimize their size and weight
*The optical filters and dichroic mirror are custom cut to minimize their size and weight
+
* A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera
*A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera.
+
* Adjustment of focal plane is done by moving imaging sensor not objective
*Adjustment of focal plane is done by moving imaging sensor not objective
 
  
  

Revision as of 13:35, 16 February 2015

UCLA MINIscope Resource

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Overview

Our miniature fluorescence microscope uses wide-field fluorescence imaging to record neural activity and network structure in awake, freely behavin mice. The functional design of our microscope is similar to a table top wide-field fluorescence microscope with the following key differences:

  • All nonessential components are removed
  • The table top objective is replaced with an imaging objective GRadient Index of Refraction (GRIN) lens
  • The excitation light source is a super bright LED rather than a lamp or laser
  • The optical filters and dichroic mirror are custom cut to minimize their size and weight
  • A small CMOS imaging sensor is used to record emission light instead of a large CCD, emCCD, or sCMOS camera
  • Adjustment of focal plane is done by moving imaging sensor not objective


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