I have been trying to process some of my recordings, but it seems that the alignment causes more harm than good, when there is no clear landmark visible.
I tried it with the Subiculum example data set and I have a similar issue.
If one calculates the max projection before and after alignment, there are extra "noise" bright spots that pop-up for the aligned case.
From what I understand, the idea is that you are supposed to use as ROI an area with some visible landmark (e.g. vessels) but ideally no cells and no dust/spots.
Has anybody experienced something similar? Any solutions/ideas?
|Posted by Nikolas on 25 June 2016 at 15:21.|